Light and nanotechnology combine to prevent biofilms on medical implants

Institute of Photonic Sciences (ICFO)
  • ICFO and B. Braun Surgical develop a new technique that could prevent bacterial contamination and infection in medical implants
  • The procedure generates heat via the application of light radiation onto gold nanoparticles on the implants
  • This advance may contribute to avoid post-surgery complications, keeping minor surgeries as safe as possible.

 

On surgical meshes, caveats, and how to avoid them

Invented approximately 50 years ago, surgical medical meshes have become key elements in the recovery procedures of damaged-tissue surgeries, the most frequent being hernia repair. When implanted within the tissue of the patient, the flexible and conformable design of these meshes helps hold muscles tight and allows patients to recover much faster than through the conventional sowing and stitching surgery.  

However, the insertion of a medical implant in a patient’s body carries alongside the risk of bacterial contamination during surgery and subsequent formation of an infectious biofilm over the surface of the surgical mesh. Such biofilms tend to act like an impermeable coating, impeding any sort of antibiotic agent to reach and attack the bacteria formed on the film in order to stop the infection. Thus, antibiotic therapies, which are time-limited, could fail against these strongly entrenched bacteria and the patient could end up in recurring surgeries that could even lead to death. As a matter of fact, according to the European Antimicrobial Resistance Surveillance Network (EARS-Net), in 2015 more than 30,000 deaths in Europe were linked to infections with antibiotic-resistant bacteria.

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Surgical implants, covered with gold nanoparticles (left) compared to the original meshes previous to the treatment (right).

In the past, several approaches have been sought to prevent implant contamination during surgery. Post-surgery aseptic protocols have been established and implemented to fight these antibiotic-resistant bacteria but none have entirely fulfilled the role of solving this issue.

In a study published in Nano Letters and highlighted in Nature Photonics, ICFO researchers Dr. Ignacio de Miguel, Arantxa Albornoz, led by ICREA Prof. at ICFO Romain Quidant, in collaboration with researchers Irene Prieto, Dr. Vanesa Sanz, Dr. Christine Weis and Dr. Pau Turon from the major medical device and pharmaceutical device company B. Braun, have devised a novel technique that uses nanotechnology and photonics to dramatically improve the performance of medical meshes for surgical implants.

 

ICFO hand in hand with B. Braun Surgical

Through an ongoing collaboration since 2012, the team of researchers at ICFO and B. Braun Surgical, S.A., developed a medical mesh with a particular feature: the surface of the mesh was chemically modified to anchor millions of gold nanoparticles. Why? Because gold nanoparticles have been proven to very efficiently convert light into heat at very localized regions.

The technique of using gold nanoparticles in light-heat conversion processes had already been tested in cancer treatments in previous studies. Even more, at ICFO this technique had been implemented in several previous studies supported by the Cellex Foundation, thus being another salient example of how early visionary philanthropic support addressed at tackling fundamental problems eventually leads to important practical applications. For this particular case, in knowing that more than 20 million hernia repair operations take place every year around the world, they believed this method could reduce the medical costs in recurrent operations while eliminating the expensive and ineffective antibiotic treatments that are currently being employed to tackle this problem.

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Scanning electron microscope (SEM) micrographs of the Staphylococcus aureus biofilm formed on the surgical mesh surface.

Thus, in their in-vitro experiment and through a thorough process, the team coated the surgical mesh with millions of gold nanoparticles, uniformly spreading them over the entire structure. They tested the meshes to ensure the long-term stability of the particles, the non-degradation of the material, and the non-detachment or release of nanoparticles into the surrounding environment (flask). They were able to observe a homogenous distribution of the nanoparticles over the structure using a scanning electron microscope.

Once the modified mesh was ready, the team prepared it for testing by exposing it to S.aureus bacteria for 24 hours until they observed the formation of a biofilm on the surface. With the biofilm yet formed, they exposed the modified mesh to twenty short intense pulses of near infrared light (800 nm) at a fixed angle of incidence, resulting in an effective bacterial removal effect.

The illumination of the mesh with the specified near-infrared light frequency would induce a phenomenon of localized surface plasmon resonance in the nanoparticles. By this effect, electrons from the metal surface become excited, resulting in the efficient conversion of light into heat, in turn resulting in the hyperthermia of bacterial cells and the biofilm on the surface of the nanoparticles.

Secondly, by using a fluorescence confocal microscope, researchers saw how much of the bacteria had died or was still alive. For the bacteria that remained alive, they observed that the biofilm bacteria became planktonic cells, recovering their sensitivity or weakness towards antibiotic therapy and to immune system response. For the dead bacteria, they observed that upon increasing the amount of light delivered to the surface of the mesh, the bacteria would increasingly lose their adherence and eventually peel off the surface.

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Schematic view of plasmon-enabled biofilm prevention on surgical meshes.

Thirdly, the researchers confirmed that operating at near infrared light ranges was completely compatible with in-vivo settings, meaning that such a technique would most probably not damage the surrounding healthy tissue. Finally, they repeated the treatment and confirmed that the recurrent heating of the mesh had not affected its light-to-heat conversion capabilities.

ICREA Prof. at ICFO Romain Quidant comments, “the results of this study have paved the way towards using plasmon nanotechnologies to prevent the formation of bacterial biofilm at the surface of surgical implants. There are still several issues that need to be addressed but it is important to emphasize that such a technique will indeed signify a radical change in operation procedures and further patient post recovery.”

As additionally explains Dr. Pau Turon, Director of Research and Development at B. Braun Surgical, S.A., “our commitment to help healthcare professionals to avoid hospital related infections pushes us to develop new strategies to fight bacteria and biofilms. Additionally, the research team is exploring to extend such technology to other sectors where biofilms must be avoided.”

In a context of increasing bacterial resistance to antibiotics, this advance may contribute to the saving of many lives from unnecessary post-surgery complications, helping to keep relatively minor surgeries as safe as possible.

 

Image credits:

All images kindly provided by ICFO.

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