We are seeking a highly motivated researcher to develop next-generation neuronal actuators based on light-gated ion channels. The ideal candidate will have experience in protein engineering, preferably involving membrane proteins and channelrhodopsins. The project will combine in silico mutagenesis with structure-guided design to identify key residues that enhance light sensitivity and photocurrent amplitude, inspired by naturally occurring photoreceptors in photosynthetic organisms. Engineered variants will be functionally characterized using in vitro reconstitution and automated patch-clamp electrophysiology.

The goal of this position is to engineer novel light-gated actuator with unprecedented photon-sensitivity for use in non-invasive, translational neurotherapies.

The position offers strong opportunities for scientific leadership and career development. The position is placed at the interface between the Prof. Dr. Michael Krieg and Prof. Dr. Nicoletta Liguori labs at ICFO and the candidate will collaborate with the two teams and thus benefit from a cutting-edge interdisciplinary environment. The candidate will be mentored by the PI and collaborators with the goal of developing the skills required to become an independent researcher.

Key Responsibilities

The successful candidate will lead a team of bioengineers, protein biochemists, and ultrafast spectroscopists to develop, deploy, and elucidate the mechanisms of novel light-gated ion channels. Working closely with team members and collaborators specializing in rational protein engineering, protein reconstitution, ultrafast spectroscopy, and C. elegans neurobiology, the candidate will:

- Engineer new channelrhodopsin variants incorporating secondary light-absorbing groups.
- Design and execute in vitro reconstitution experiments with purified proteins and targeted mutants.
- Perform electrophysiological measurements using automated patch-clamp systems to characterize photocurrents of engineered channels in heterologous expression systems.
- Collaborate with cell biologists and electrophysiologists to translate in vitro findings to heterologous cells and C. elegans neurons.

The candidate is expected to communicate results in group meetings, conferences, and peer-reviewed publications, and to provide regular updates to the PI and collaborating partners. A proven track record of effective teamwork is essential.